. 2021 Jun 1;12:665776. doi: 10.3389/fmicb.2021.665776

TABLE 2.

Kruskal-Wallis tests of SIMPER-implicated taxa.

Amplicon	Testing Block	Implicating contrast(s)	OTU	Classification	Pre Mean ± SE	Post1 Mean ± SE	Post2 Mean ± SE	χ²_df=2	P-value
Bacteria	HE Liquids	Pre/Post1	B_OTU 19	Prevotellaceae; Prevotella_1	55.6 ± 8.2^a	139.7 ± 15.1	75.2 ± 10.4^a	16.349	<0.001
		Pre/Post1, Post1/Post2	B_OTU 3	Prevotellaceae; Prevotella_1	322.3 ± 27.3^a	414.3 ± 29.4	320.2 ± 20.5^a	6.953	0.031
			B_OTU 4	Prevotellaceae; Prevotella_1	195.6 ± 27.0^a	377.8 ± 36.8	232.6 ± 25.4^a	12.297	0.002
			B_OTU 5	Prevotellaceae; Prevotella_1	208.3 ± 33.0^a	374.3 ± 36.5	235.2 ± 34.2^a	9.669	0.007
			B_OTU 6	Prevotellaceae; Prevotella_1	153.0 ± 22.9^a	268.1 ± 24.6	166.0 ± 20.2^a	10.526	0.005
		Post1/Post2	B_OTU 1	Succinivibrionaceae; Succinivibrionaceae UCG-001	1904.6 ± 301.4^a,b	1181.3 ± 362.6^a	2153.0 ± 291.9^b	6.314	0.043
			B_OTU 2	Prevotellaceae; Prevotella_1	653.5 ± 77.8^a	759.8 ± 53.9^a	593.2 ± 59.9^a	5.012	0.082
			B_OTU 29	Prevotellaceae; Prevotella_7	145.7 ± 33.6^a	119.6 ± 55.8^a	251.1 ± 46.7^a	7.214	0.027
			B_OTU 52	Prevotellaceae; Prevotella_7	115.7 ± 30.7^a,b	44.3 ± 15.8^a	132.9 ± 24.4^b	5.870	0.053
	HE Solids	Pre/Post1, Post1/Post2	B_OTU 18	Lachnospiraceae; Butyrivibrio_2	61.4 ± 8.6^a	124.0 ± 19.7	62.5 ± 6.6^a	10.512	0.005
		Post1/Post2	B_OTU 7	Succinivibrionaceae; Succinivibrionaceae UCG-002	56.3 ± 19.2^a	151.5 ± 35.0	62.2 ± 21.9^a	7.181	0.028
			B_OTU 20	Lachnospiraceae; Oribacterium	175.9 ± 35.0^a,b	101.0 ± 39.9^a	186.5 ± 29.2^b	7.190	0.027
	LE Liquids	Post1/Post2	B_OTU 1	Succinivibrionaceae; Succinivibrionaceae UCG-001	690.4 ± 205.6^a,b	1106.7 ± 259.7^a	422.1 ± 147.2^b	6.464	0.039
			B_OTU 3	Prevotellaceae; Prevotella_1	423.9 ± 26.3^a,b	367.7 ± 23.5^a	462.8 ± 27.6^b	6.930	0.031
			B_OTU 4	Prevotellaceae; Prevotella_1	359.6 ± 27.3^a	261.3 ± 28.7	426.9 ± 36.7^a	11.256	0.004
			B_OTU 5	Prevotellaceae; Prevotella_1	398.8 ± 33.1^a	278.2 ± 35.1	462.3 ± 31.8^a	12.875	0.002
			B_OTU 27	Prevotellaceae; Prevotella_7	37.5 ± 23.1^a	103.1 ± 36.4^a	33.0 ± 27.2	13.348	0.001
			B_OTU 29	Prevotellaceae; Prevotella_7	38.1 ± 23.8^a	112.0 ± 44.5	47.0 ± 35.3^a	12.078	0.002
			B_OTU 52	Prevotellaceae; Prevotella_7	33.9 ± 21.4^a	74.8 ± 26.6	20.2 ± 15.4^a	14.165	<0.001
	LE Solids	Pre/Post1	B_OTU 5	Prevotellaceae; Prevotella_1	153.8 ± 20.7	79.4 ± 13.4^a	100.7 ± 14.6^a	9.063	0.011
		Pre/Post1, Post1/Post2	B_OTU 20	Lachnospiraceae; Oribacterium	9.8 ± 3.4^a	110.4 ± 30.2	36.8 ± 24.2^a	21.334	<0.001
Fungi	HE Liquids	Pre/Post1	F_OTU 5	Neocallimastigaceae; Neocallimastix	141.2 ± 22.5^a	61.5 ± 8.3^b	117.0 ± 23.0^a,b	9.305	0.010
		Pre/Post1, Post1/Post2	F_OTU 7	Saccharomycetales Incertae sedis; Wickerhamomyces anomalus	149.1 ± 29.7^a	45.0 ± 19.3	170.0 ± 48.4^a	14.854	<0.001
		Post1/Post2	F_OTU 2	Neocallimastigaceae; unclassified	277.9 ± 46.2^a	513.8 ± 74.2	239.4 ± 38.9^a	9.678	0.008
			F_OTU 9	Neocallimastigaceae; Piromyces	44.6 ± 13.2^a,b	44.3 ± 7.5^a	20.1 ± 3.8^b	6.436	0.040
	HE Solids	Pre/Post1	F_OTU 5	Neocallimastigaceae; Neocallimastix	209.9 ± 28.4	75.3 ± 7.9^a	111.3 ± 17.3^a	20.020	<0.001
			F_OTU 6	Neocallimastigaceae; Neocallimastix	88.1 ± 14.3^a	145.5 ± 8.1^b	121.0 ± 13.8^a,b	7.192	0.027
			F_OTU 10	Saccharomycetales Incertae sedis; Debaryomyces prosopidis	64.2 ± 36.7^a	5.1 ± 3.3	15.6 ± 5.1^a	9.556	0.008
		Pre/Post1, Post1/Post2	F_OTU 2	Neocallimastigaceae; unclassified	310.5 ± 62.8^a	518.9 ± 52.4	242.6 ± 60.8^a	10.504	0.005
			F_OTU 3	Neocallimastigaceae; Piromyces	67.5 ± 23.0^a	92.3 ± 13.8	41.1 ± 13.1^a	9.950	0.007
			F_OTU 7	Saccharomycetales Incertae sedis; Wickerhamomyces anomalus	141.1 ± 68.6^a	7.6 ± 1.9	238.4 ± 84.9^a	18.930	<0.001
		Post1/Post2	F_OTU 4	Neocallimastigaceae; Piromyces	182.6 ± 28.7^a,b	186.9 ± 14.9^a	110.4 ± 22.7^b	7.749	0.021
			F_OTU 11	Trichocomaceae; Penicillium roqueforti	2.9 ± 1.6^a	0.3 ± 0.2^a	173.0 ± 81.7	32.528	<0.001
	LE Liquids	Pre/Post1, Post1/Post2	F_OTU 5	Neocallimastigaceae; Neocallimastix	62.4 ± 23.5^a	158.2 ± 21.0^b	71.3 ± 8.4^c	19.725	<0.001
		Post1/Post2	F_OTU 4	Neocallimastigaceae; Piromyces	323.0 ± 31.6^a	387.4 ± 68.3^a	147.2 ± 16.9	19.223	<0.001
			F_OTU 7	Saccharomycetales Incertae sedis; Wickerhamomyces anomalus	54.8 ± 11.4^a	24.2 ± 5.4	105.2 ± 26.3^a	9.436	0.009
			F_OTU 11	Trichocomaceae; Penicillium roqueforti	1.3 ± 0.7^a	0.1 ± 0.1^a	44.0 ± 15.6	31.621	<0.001
	LE Solids	Pre/Post1	F_OTU 5	Neocallimastigaceae; Neocallimastix	64.3 ± 8.7^a	199.7 ± 26.8^b	120.6 ± 11.7^c	22.600	<0.001
		Pre/Post1, Post1/Post2	F_OTU 7	Saccharomycetales Incertae sedis; Wickerhamomyces anomalus	37.6 ± 12.7^a	8.7 ± 3.8	32.4 ± 13.6^a	11.572	0.003
		Post1/Post2	F_OTU 4	Neocallimastigaceae; Piromyces	252.2 ± 36.4^a,b	298.1 ± 46.2^a	168.6 ± 18.3^b	7.687	0.021
			F_OTU 11	Trichocomaceae; Penicillium roqueforti	1.0 ± 0.3^a	0.1 ± 0.1^b	112.0 ± 96.4^c	26.006	<0.001

Tests performed within amplicon, sample type, and efficiency status. Post hoc testing was performed as Wilcoxon rank sum tests with FDR correction applied to P-values. Means sharing letters are not significantly different (α = 0.05). All samples normalized to prior to analysis (bacteria: 10,000 sequences/sample, fungi: 2,450 sequences/sample).