Figure 3.

Effects of somatotroph-selective GHSR deletion on acyl-ghrelin bolus-induced GH secretion, food intake, and blood glucose. (A) Plasma acyl-ghrelin measured at baseline and 30 min post-ghrelin (1 mg/kg BW, i.p.) administration. n = 13–18. Plasma GH measured at baseline (B) and 5 min after and (C) 15 min after acyl-ghrelin treatment. n = 4–8. Food intake measured at (D) 45 min, (E) 90 min, (F) 4 h, and (G) 24 h after acyl-ghrelin (1 mg/kg BW, s.c.) injection. n = 11–18 (including n = 3 female + 10 male GHSR^WT/WT/GH^WT/WT mice; n = 4 female + 7 male GHSR^WT/WT/GH^WT/iCre mice; n = 5 female + 7 male GHSR^fl/fl/GH^WT/WT mice; n = 8 female + 10 male GHSR^fl/fl/GH^WT/iCre mice). (H) Blood glucose measured after 30 min following acyl-ghrelin (1 mg/kg BW, s.c.) treatment. n = 13–18. Data in A-C and H were analyzed by repeated-measures two-way ANOVA followed by Sidak's multiple comparisons test. Data in D-G were analyzed by two-way ANOVA followed by Sidak's multiple comparisons test. All of the values are expressed as mean ± SEM. ∗P < 0.05, ∗∗P < 0.005, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001 for significant changes or ns = no significant changes.