HEPES buffer (pH 7.3 or 7.5)
| Reagent | Final concentration | Amount |
|---|---|---|
| Hepes | 1 M | 238.3 g |
| ddH2O | n/a | to 1 L |
| Total | n/a | 1 L |
It is good for 6 months at 25°C.
pH adjust this solution using NaOH
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