Figure 3. Results of analysis of subcellular localization of GFP fusion proteins in tobacco protoplasts isolated from the cells of an agroinfiltrated leaf area.

. Transient gene expression of plant expression vectors, with leader signal sequences providing their localization in various compartments of plant cells, demonstrates that GFP fusion protein is localized to the chloroplasts (I), endoplasmic reticulum (II), and cytoplasm (III). The merged images (first column) include the green channel (last column) and the chloroplast autofluorescence channel (middle column). Scale bars = 10 μm.