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. 2018 Feb 5;8(3):e2712. doi: 10.21769/BioProtoc.2712

Figure 1. Postnatal astrocyte culture.

Figure 1.

Upper scheme illustrates the main steps for cortical and cerebellar astrocyte culture. Red dashed lines represent approximate regions of cuts. Lower left panels (A-D) show both cultures at 80% confluence and immunostained for the astrocytic marker GFAP (red) and neuronal marker BIII-tubulin (green). Quantifications show that > 97% of the cells in both cultures are GFAP+/BIII-tubulin- (E). Cereb = cerebellum, Ctx = cortex, Astro = astrocytes. Nuclei are stained with DAPI (blue), scale bars = 25 µm.