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Upper scheme illustrates the principal steps of astroglial nucleofection using the 4D nucleofector (LONZA). Lower panels show an example of cerebellum astrocytes (GFAP+) 24 h after nucleofection with pCAG-Ascl1-IRES-DsRed (A). Astrocytes express reporter fluorescence (RFP+) (white arrows) (B, C). Scale bars = 25 µm.
