Table 1. GLC operating conditions for determination of fatty acid composition.
| Specification | |
| Column | ULBON HR-Thermon-3000B 0.25 x 25 m |
| Injection volume | 1 μl |
| Carrier gas flow | 1 ml/min Helium, 115 kPa |
| Injector temperature | 250 °C, split |
| Split flow | 50 ml/min (split ratio: 1:50, v/v) |
| Oven temperature | 180 °C isothermal |
| Detector | 250 °C, Flame Ionization Detector (FID) |
Note: FID detects ions that are generated through burning of carbon compounds (Holm, 1999).