Figure 5.

Breaking the NeutrAvidin-streptavidin linkage by 7 M NaOH. (A and B) Schematic diagram of PEG-passivated slide coated with Alexa-Fluor-555-labeled streptavidin (Thermo Fisher Scientific) and detachment by 7 M NaOH treatment. (C) Representative fields of view before and after treatment of Alexa-Fluor-555-labeled streptavidin (Thermo Fisher Scientific) and 7 M NaOH, respectively. (D) Molecule count during each repeat of binding and unbinding trials (left side). Shown is the molecule count of Alexa-Fluor-555-streptavidin (Thermo Fisher Scientific) bound on surface before and after 8 M GdmCl and 10% SDS treatment. Error bar represents the SD from molecule counts of 20 different field of view. To see this figure in color, go online.