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Antibody-bound surface regeneration. (A) Schematic diagram of Cy3-U50 engaged with FUS immobilized to the surface via (A) anti-GFP antibody, (D) anti-histidine antibody, and (F) anti-MBP antibody and regeneration strategies by NaOH and SDS. (B, E, and G) Normalized molecule count during each repeat of experiments with 0.1% SDS and (C) 7 M NaOH treatment. All error bars represent the SD from molecule counts of 20 different field of view. To see this figure in color, go online.
