Figure 6. CXCR6⁻ T_Eff/EMs are the precursor of distant T_RMs.

(A) Experimental design of sample collection for TCR-β repertoire sequencing.

(B) Repertoire analysis shows substantial overlap between high and low frequency TCRs between tumor and distant T_RMs.

(C and D) Repertoire analysis shows that both CXCR6^NEG and CXCR6^POS subsets share TCRs with tumor T_RMs; only the highly expanded clones in the CXCR6^NEG population contributed to distant T_RM formation.

(E) Experimental design for T_Eff/EM precursor transfer.

(F) Preference of CXCR6⁺ T_Eff/EM precursor cells to stay in the tumor while CXCR6⁻ T_Eff/EM precursors egress to the distant mucosa. Data are represented as a ratio of recovered cells in the tumor divided by the recovered cells in the distant mucosa or LN. Mann-Whitney test, p < 0.05. Bars represent means ± SEMs and symbols represent individual mice. Data are pooled over 3 independent experiments.