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. 2021 Jun 1;11:669989. doi: 10.3389/fcimb.2021.669989

Figure 2.

Figure 2

P.gingivalis impairs maturation of bone marrow derived dendritic cells(DCs) from young and old mice. At day 6, DCs from young (yDCs) and old (oDCs) mice were co-cultured with P.gingivalis (10 MOI) ± Rapamycin (1µM), Pg LPS (100ng/ml) or Ecoli LPS (100ng/ml). Cells were harvested at day 8 and analyzed by flow cytometry for the expression of maturation markers, MHCII and co stimulatory molecules CD80 and CD86. (A) Flow cytometry scatter gram and its corresponding bar graph showing % of MHCII positive cells in young (upper panel) and old (lower panel), DCs gated on CD11c+ cells (gate not shown). (B) Flow cytometry scatter gram and its corresponding bar graph showing % of CD86 positive cells in young (upper panel) and old (lower panel), DCs gated on CD11c+ cells (gate not shown).(C) Flow cytometry scatter gram and its corresponding bar graph showing % of CD80 positive cells in young (upper panel) and old (lower panel), DCs gated on CD11c+ cells (gate not shown). Analysis was done using Two-way ANOVA and Dunn’s correction test. (Data are expressed as means ± SD, *p<0.05, **p<0.01, ***p<0.001). NS, not significant.