Figure 3.
Milk-derived ZAC-3 hIgA1 Reduces Intestinal Colonization of V. cholerae O1. CD-1 pups, 4 to 5 days old, nursed from WT or ZAC-3 hIgA-positive transgenic dams were gavaged with 1 × 107 V. cholerae (A) O395 or (B) C6706 cells. After 24 hours incubation pups were euthanized and whole intestines were plated for CFUs. There is a significant reduction in log CFUs in pups that were fed from dams secreting ZAC-3 hIgA in comparison to WT dams, for both strains of V. cholerae O1. Experiments were performed on 2 litters for hIgA positive dams, and 6 times on WT dams, with 2 to 4 pups per experimental group, each dot represents the intestinal CFUs of a single pup. Statistical significance was determined using Student's t-test. P values are indicated on graph.