Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 8;5(1):89–116. doi: 10.20411/pai.v5i1.370

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Pathogens and Immunity 2020

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Supplementary Figure 6. — Western blots confirm the presence of HC, LC, and J chain. Milk samples from control mice secreting HUMIRA IgA, previously unpublished, or ZAC-3 hIgA1 founder females, numbers 144, 21, 59 containing the BC3239 and BC3242 constructs were analyzed via western blot for (A) Kappa light chain and (B) human IgA. Lanes 1, 2, containing positive control Human IgA and lane 5, containing milk from founder female 21, exhibit highly positive signal for both Kappa LC and IgA. (C) Western blot showing that milk from mouse 21 is also positive for J-chain in comparison to positive controls. Data in (C) also includes preliminary findings from ZAC-3 IgA2 constructs, mouse numbers 14, 15, 16, 51, which were not pursued. ZAC-3 hIgA founder female 21 was subsequently bred.