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. 2020 May 8;5(1):89–116. doi: 10.20411/pai.v5i1.370

Supplementary Table 3.

List of plasmids utilized in this work.

Plasmid Name Characteristics/Sequence Source/Reference
pBC1 Contains goat β-casein expression cassette Invitrogen
BC451 Beta Casein expression vector encoding a 7.2kb downstream sequence including the last 3 exons of beta casein (X. Yu et al. 2013)
BC350 Beta Casein expression vector encoding a 7.2kb downstream sequence including the last 3 exons of beta casein (X. Yu et al. 2013)
BC2797 Beta Casein expression vector encoding a 300bp bgH polyA sequence (X. Yu et al. 2013)
BC3242 BC451 encoding ZAC-3 IgA1 HC (Figure S1) This work
BC3235 BC2797 encoding ZAC-3 IgA1 LC (Figure S2) This work
BC2635 BC350 encoding J chain (Figure S3) This work
BC3239 Notl-Sall fragment of BC3235 and Sall-NotI fragment of BC2635 ligated into Supercos (Figure S4) This work
pMQCherry80 mCherry open reading frame Matthew Wargo, unpublished
pUC18-mTn7T-eyfp-Gm constitutive derivative of the Plac promoter (PA/01/04/03) [33]
pGW104 pUC18T-mTn7T-eyfp with constitutive Plac promotor and mCherry ORF This work