Supplementary Table 3.
List of plasmids utilized in this work.
Plasmid Name | Characteristics/Sequence | Source/Reference |
---|---|---|
pBC1 | Contains goat β-casein expression cassette | Invitrogen |
BC451 | Beta Casein expression vector encoding a 7.2kb downstream sequence including the last 3 exons of beta casein | (X. Yu et al. 2013) |
BC350 | Beta Casein expression vector encoding a 7.2kb downstream sequence including the last 3 exons of beta casein | (X. Yu et al. 2013) |
BC2797 | Beta Casein expression vector encoding a 300bp bgH polyA sequence | (X. Yu et al. 2013) |
BC3242 | BC451 encoding ZAC-3 IgA1 HC (Figure S1) | This work |
BC3235 | BC2797 encoding ZAC-3 IgA1 LC (Figure S2) | This work |
BC2635 | BC350 encoding J chain (Figure S3) | This work |
BC3239 | Notl-Sall fragment of BC3235 and Sall-NotI fragment of BC2635 ligated into Supercos (Figure S4) | This work |
pMQCherry80 | mCherry open reading frame | Matthew Wargo, unpublished |
pUC18-mTn7T-eyfp-Gm | constitutive derivative of the Plac promoter (PA/01/04/03) | [33] |
pGW104 | pUC18T-mTn7T-eyfp with constitutive Plac promotor and mCherry ORF | This work |