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. 2021 Mar 17;120(10):1927–1941. doi: 10.1016/j.bpj.2021.03.010

Figure 3.

Figure 3

The inter-FRET signal from BongwooriR3-derived GEVIs. Voltage-induced optical response of HEK cells expressing BongwooriR3-CFP (A) or Bongwoori-YFP (B). (C) Optical response from HEK cells coexpressing BongwooriR3-Cerulean and BongwooriR3-Venus. (D) The trace is from a hippocampal neuron in culture under whole-cell current clamp coexpressing BongwooriR3-Cerulean and BongwooriR3-Venus. The black trace is voltage. The blue trace is CFP emission at 480 nm. The red trace is from YFP emission at 540 nm. Excitation wavelength is 430 nm. The green trace is the ratio of YFP/CFP fluorescence. Inset is a time expanded view of the first action potential.