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Monomeric mutations to the FP domains may reduce but do not destroy the voltage-dependent, intermolecular FRET signal. The top trace is the intermolecular FRET signal reproduced from Fig. 2C for comparison purposes. The bottom three traces are from HEK cells expressing ArcLight inter- FRET pairs containing one of the three FP monomeric mutations (A206K, L221K, or F223R). The dark trace is the mean from at least four cells. The shaded area is the SE of the mean.
