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. 2021 Mar 17;120(10):1927–1941. doi: 10.1016/j.bpj.2021.03.010

Figure 7.

Figure 7

Intermolecular FRET with a farnesylated FP. (A) CFP fused to the voltage-sensing domain of the GEVI Bongwoori-R3 was coexpressed in HEK cells with a farnesylated version of YFP. The blue trace is the emission at 480 nm. The red trace is the emission at 540 nm. The excitation wavelength was 430 nm. Dark line is the mean of six cells. Shaded areas are the SE of the mean. (B) YFP fused to the voltage-sensing domain of Bongwoori-R3 was coexpressed with a farnesylated CFP. The color coding and wavelengths are as in (A). (C) The FP mRuby2 was fused to the GEVI ArcLight and coexpressed with a farnesylated Clover FP. The green trace is the emission at 520 nm. The red trace is the emission at 585 nm. The excitation wavelength is 470 nm. (D) Intermolecular FRET of a rhodopsin GEVI, Ace_2N (27), lacking the mNeon FP was coexpressed with farnesylated-Clover.