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. 2021 Mar 9;120(9):1705–1717. doi: 10.1016/j.bpj.2021.02.044

Figure 2.

Figure 2

Cytoskeletal activity is correlated with changes in cellular energetics in 2D confinement. (A) The color-coded time-lapse of MDA-MB-231 membrane ruffling is shown. (B) Normalized stepwise cell size change and normalized PercevalHR ratio change of single MDA-MB-231 cell spreading on 22.4 × 22.4 μm, 50 × 10 μm, and 5 × 100 μm collagen micropatterns are shown. (C) Cross correlation (XCF) of single-cell normalized PercevalHR ratio and cell membrane ruffling for cells with increasing elongation (n = 20 cells) is shown. Total membrane ruffling as a function of total normalized PercevalHR ratio (D) averaged for each pattern and for (E) individual control cells is shown (n = 41 cells). Dashed yellow squares show zoomed regions; dashed red lines show one-tailed t-test significance; lines show LOWESS smoothing, and dashed lines show linear regression; ∗p < 0.05. Scale bars, 25 μm. To see this figure in color, go online.