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. 2021 Mar 9;120(9):1705–1717. doi: 10.1016/j.bpj.2021.02.044

Figure 3.

Figure 3

Inhibition of MDA-MB-231 OXPHOS lowers cellular energetics and cytoskeletal activity in 2D confinement. (A) The color-coded time-lapse of membrane ruffling of MDA-MB-231 cells after AMA treatment on collagen micropatterns with increasing elongation is shown (dashed squares show zoomed regions; yellow, ctrl; red, AMA). Quantification of (B) normalized PercevalHR ratio (n = 55–64 cells) and (C) membrane ruffling (n = 30–35 cells) after AMA treatment on collagen micropatterns with increasing elongation is shown. (D) Total change in membrane area as a function of normalized PercevalHR ratio averaged for each pattern after AMA treatment is shown (p shows extra-sum-of-squares F-test that compares linear regression). Data shown as box-and-whisker plots denote mean (+), medians and 25th/75th and 5th/95th percentiles, or mean ± SE; dashed lines show linear regression; two-tailed t-test was used when comparing between ctrl and AMA, and multiple comparisons was used with ANOVA when comparing across patterns; n.s., nonsignificant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Scale bars, 25 μm. To see this figure in color, go online.