Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 20;120(9):1846–1855. doi: 10.1016/j.bpj.2021.03.030

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Biophysical Society.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Structure of pRab8a:RL2¹¹⁷ complex reveals Arg/Arg stacking. (A) Domain organization of RILPL2 and Rab8a is shown. The N-terminal RH1 domain of RILPL2 binds to myosin Va. The hinge region between RH1 and RH2 domains is predicted to be flexible from secondary structure analyses. (B) Overall view of the pRab8a:RL2¹¹⁷ heterotetramer is shown. Dashed rectangle is the X-cap that is seen up close in (C) and (D). Cyan sphere is an Mg²⁺ ion that interacts with GTP and switch 1 of pRab8a. (C) Left: structure of pRab8a:RL2¹²⁹ in the region of X-cap. H128^RL2 is a non-native residue that comes from the hexahistidine affinity tag. Right: structure of pRab8a:RL2¹¹⁷ at the X-cap is shown. R130^RL2 stacks against the guanidino group of R132^RL2. Comparisons of arginine conformations at the interfaces are highlighted with the shaded circle for complexes in (B) and (C). To see this figure in color, go online.