Fig. 4.

TRM clones exhibit tissue-specific expansions and clonal stability with age. A Clone tracking across sites and subsets. Each line-circle plot depicts and individual CD4⁺ or CD8⁺ T cell clone, their subset identity and relative frequency in each site. Each line represents a single clone, circles indicate presence within a site (bone marrow (BM), blood (BL), lung-draining lymph node (LN), lung (LG), or spleen (SP)); the size of the circle is proportional to the total number of copies of the clone in the sample, and colored wedges in the circle indicate what fraction of copies derive from individual subsets (TCM (blue), TEM (orange), TEMRA (green), TRM (red)) from CD4⁺ (left) and CD8⁺ (right) T cells for D324 (top), D383 (middle) and D466 (bottom). Representative clones shown from the top 500 clones for each donor. B Clonality associations with age for each subset. Scatter plots with fitted lines (black) of clonality measurements from Fig. 1 by age from linear regression analysis with associated p values (P) indicated below each plot for CD4⁺ (left) and CD8⁺(right) subsets. Tissue site indicated by shape