Fig. 5.

MiR-1205 bound to GRIK3 in CRC cells. A The binding sequence between miR-1205 and GRIK3 was assessed by miRDB online database. B The binding relationship between miR-1205 and GRIK3 was confirmed by dual-luciferase reporter assay. C MiR-1205 inhibitor upregulated GRIK3 protein expression, and miR-1205 mimic downregulated GRIK3 protein expression. D–F GRIK3 expression was upregulated in CRC tissues as well as SW480 and SW620 cells. Significant differences in (B and E) were compared with two-tailed Student’s t tests, in (C and F) with ANOVA and in (D) with Wilcoxon rank-sum test. *P < 0.05