Fig. 6.

MiR-1205 mimic suppressed CRC development via interacting with GRIK3. A The high efficiency of pcDNA-GRIK3 in increasing GRIK3 protein expression was determined by western blot in SW480 and SW620 cells. B GRIK3 overexpression attenuated miR-1205-mediated repression on the colony-forming ability of SW480 and SW620 cells. C, D The effects of miR-1205 mimic on cell cycle process and cell apoptosis were hindered by ectopic GRIK3 expression. E The effects of miR-1205 on the protein expression of Bcl-2 and Bax were impaired by GRIK3 reintroduction. F–H MiR-1205-triggered inhibition on cell migration and invasion was reversed after GRIK3 overexpression. The P value in (A) were calculated with two-tailed Student’s t tests and in (B–H) with ANOVA. *P < 0.05