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. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: J Neurosci Methods. 2011 Dec 13;204(2):389–397. doi: 10.1016/j.jneumeth.2011.11.031

Figure 2. Framework overview.

Figure 2.

A brain region is selected for targeting and a virtual chamber is placed. Several external markers are rigidly attached to the skull by drilling into an existing implant or securing a small attachment to the head post (not shown). Marker positions in the MR image is identified. During the surgical procedure the position of the markers is read out using the stereotactic manipulator. This can be done even if the stereotactic frame is not physically attached to the animal, but instead to the primate chair. The software solves the targeting problem given the read out values and outputs the set of parameters that are needed to align the manipulator with the planned virtual chamber position.