Figure 1 . RPLP1 expression is elevated in human ectopic lesion tissue and localised in the glandular epithelium. (A) RPLP1 was immunohistochemically localised in ectopic endometriotic tissue and matched eutopic tissues from women with confirmed endometriosis, and eutopic control from women without endometriosis during the proliferative (upper panel) and secretory (lower panel) stages of the menstrual cycle. The arrow illustrates the location of RPLP1 protein in the glandular epithelium of the ectopic tissues (where the brown colour indicates positive staining). All magnifications were ×20 (scale bar indicates 50 μm), except for proliferative ectopic endometriosis which was ×10 (scale bar indicates 100 μm). Negative, isotype-matched control is depicted in the far-right panel at ×20 magnification (scale bar indicates 50 μm). (B) Immunohistochemical histological score (H-Score) of RPLP1 in endometrial tissues (ectopic and eutopic) in both stages of menstrual cycle. Data are presented as the mean + SEM and were analysed by one-way ANOVA followed by Bonferroni post hoc analysis. Different letters indicate statistically significant different mean values, and the asterisk indicates significantly lower expression in secretory ectopic lesion tissue (Ect-Osis) compared to the proliferative ectopic lesion tissue. Data are from a total of 48 specimens, 20 control subjects (N = 10 in the proliferative and N = 10 in the secretory stage of the menstrual cycle) and 28 endometriosis subjects (N = 16 in the proliferative stage and N = 12 in the secretory stage of the menstrual cycle).