Figure 2 . RPLP1 mRNA and protein are elevated in endometriotic lesions. Endometriotic lesions and matched eutopic tissue were assessed for mRNA and protein expression of RPLP1v1 and RPLP1v2. (A) Average fold change from matched eutopic endometrium was calculated for both RPLP1v1 and v2, normalising to eutopic endometrium values. Box and whisker plots display data comparing RPLP1 mRNA variant 1 (v1) and variant 2 (v2) between eutopic and ectopic endometrial samples. Data were not normally distributed and were therefore analysed using non-parametric Mann–Whitney U tests. Both RPLP1 v1 and RPLP1 v2 transcript levels were significantly higher (P < 0.01) in ectopic lesion tissue (mean value is indicated by the X in each whisker and box plot). (B) Western blot analysis confirms expression of greater levels of RPLP1 protein expression in lesion tissue compared to matched eutopic tissue. Western blot data are representative from a total 20 matched eutopic and ectopic (lesion) samples for 8 stage I/II disease (N = 4 proliferative, N = 4 secretory stage) and 12 stage III/IV disease (N = 7 proliferative, N = 5 secretory stage). Bar graph depicts RPLP1/β-actin levels comparing eutopic and ectopic levels of expression. *P < 0.001 by unpaired t test, ectopic compared to eutopic within stage of menstrual cycle.