Figure 4 . Stable RPLP1 knockdown by lentiviral pseudoparticles results in reduction in cell survival. (A) Endometriotic epithelial cells (12Z) were infected with GFP-lentiviral pseudoparticles containing a non-targeting (control) cDNA or shRNA for RPLP1, and the cells were cultured and treated with puromycin after 48 h for positive cell selection. The cells then were then assessed for cell viability at 72 h post transfection. The total number of viable cells was assessed by manual counting of GFP-expressing cells as described in the Materials and Methods. Data are representative of three separate experiments (N = 3) which resulted from three different infections. (B) Data are displayed as the mean + SEM. Different letters indicate statistical significance (P < 0.001) between the means using unpaired t test. B depicts a representative culture of 12Z cells infected with non-targeting control shRNA and RPLP1 shRNA. The arrows depict dead/floating cells and the circle depicts a cell attached to culture plate.