Figure 6 . Immunohistochemical localisation (IHC) of RPLP1 in mouse endometrium and matched endometriotic lesion tissue. IHC was performed on eutopic uterine tissue from sham (A) and mice with experimentally-induced endometriosis (B) as well as endometriotic lesion tissue (D). All tissues were obtained during the estrogen-dominate stages (proestrus or oestrus) of the estrous cycle 2 weeks after endometriosis was induced. Arrows indicate glandular epithelium and the black ovals in B indicate glands in eutopic endometrium of mice with endometriosis which show elevated RPLP1 localisation. A and B are eutopic endometrium, C represents the entire lesion (H&E staining only) and the red box indicates the sections depicted in D and E, which represent RPLP1 antibody (1:300) staining and a negative control (isotype-matched antibody), respectively. Observations are representative of data obtained from four animals per group (N = 4). Scale bar = 100 μm in A and B.
