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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Gastroenterology. 2020 Apr 11;159(2):624–638.e9. doi: 10.1053/j.gastro.2020.03.075

Figure 6. Cholestatic and hepatocellular injury does not significantly change the location of Gli1 expression.

Figure 6.

(A) Heatmap of Gli transcription factors, showing specificity of Gli1 and Gli2 to PMCs and Gli3 to both cell types. (B) Gli3:LacZ reporter showing positive cells around the bile duct, but also stellate cells within the hepatic parenchyma (arrowheads) (n = 3). (B) 14 days after BDL, Gli1 expression is localized around the main duct and its small branches, but does not extend to the cells of the ductular reaction (arrowhead). (C) After 28 days of biweekly CCL4 injections, Gli1 expression remains around the bile duct (arrow) near the portal vein (PV). (D) Gli1 expression remains around a hilar bile duct and is not significantly induced around cells of the ductular reaction (arrowhead) after 21 days of DDC. (E) Similarly, DDC does not induce Gli1 expression within cells of the ductular reaction in peripheral ducts, but remains around the main duct (dashed outline). Scale bars, 100 μm (E and F scaled at same ratio as C).