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. 2021 Jun 1;9:680968. doi: 10.3389/fcell.2021.680968

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Copyright © 2021 Li, Zhuo, Chen, Zhang, Chen, Hu, Chen, Yang, Zhou, Mao, Xu, Ju, Shen, Wang, Dong, Xie, Zhou and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Proliferative, migratory and invasive potential of the 4Z-B-1 cell line. (A) Growth curves of 4Z-B-1 and SK-BR-3 cells determined by the CCK8 assay. (B,C) Distribution of 4Z-B-1 and SK-BR-3 cells determined by flow cytometry and statistical analysis. (D) Expression of CyclinE1 in 4Z-B-1 and SK-BR-3 cells assessed by Western blotting. (E,F) Representative micrographs and statistical analysis of migrated and invasive 4Z-B-1 and SK-BR-3 cells. (G,H) Representative micrographs and statistical analysis of the invasive area of 4Z-B-1, SK-BR-3, MCF-7, and MDA-MB-231 mammospheres in Matrigel. (I) Expression of EMT markers in 4Z-B-1, SK-BR-3, MCF-7, and MDA-MB-231 cells determined by Western blotting. ****p < 0.0001. Magnification, (E) ×100; (G) ×40.