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. 2021 May 2;40(12):e107192. doi: 10.15252/embj.2020107192

Figure 2. FOXP2 expression is regulated by flow.

Figure 2

  • A
    Whole‐mount immunofluorescence of embryonic mesenteries of the indicated developmental stages. Note induction of FOXP2 expression at E17.
  • B
    Whole‐mount immunofluorescence of E18 mesenteries fixed immediately after dissection (left panel) or after 24 h of ex vivo culture (right panel). Note loss of patterning of PROX1high valve LECs and downregulation of FOXP2 expression in flow‐abrogated vessels after ex vivo culture.
  • C, D
    Immunofluorescence (C) and qRT–PCR analysis (D) of HDLECs grown under static conditions or exposed to OSS for 48 h (n = 3 independent experiments). Data are presented as mean ± SD. P, Student's t‐test.

Data information: Scale bar: 100 µm (A, B), 50 μm (C).

Source data are available online for this figure.