Figure EV5. Contributions of stretching free energy of B blocks in the core to the structure of paraspeckles.

1. Energetic contributions of triblock copolymer micelle model of the paraspeckle considering elastic free energy of B blocks (see the Materials and Methods for details).
2. Theoretical calculation of the fraction α of the A blocks in the shell versus the length of the A blocks (represented by the number of segments) of spherical paraspeckles in the steady state with (magenta) and without (black) the stretching free energy of B blocks in the core. The parameters used for the calculations are shown in the Materials and Methods (the broken curves are results for the excluded volume $v_{\mathrm{A}}/b^3=0.5$ and $v_{\mathrm{C}}/b^3=0.5$). These data are related to Fig 4D.
3. Theoretical calculation of the radius of paraspeckles versus the length of the A blocks (represented by the number of segments) of spherical paraspeckles in the steady state with (magenta) and without (black) the stretching free energy of B blocks in the core. The radius was rescaled by the segment length. The parameters used for the calculations are shown in the Materials and Methods (the broken curves are the results for the excluded volume $v_{\mathrm{A}}/b^3=0.5$ and $v_{\mathrm{C}}/b^3=0.5$). These data are related to Fig 5A.
4. Theoretical calculation of the number of NEAT1_2 transcripts versus the length of the A blocks (represented by the number of segments) of spherical paraspeckles in the steady state with (magenta) and without (black) the stretching free energy of B blocks in the core. The parameters used for the calculations are shown in the Materials and Methods (the broken curves are the results for the excluded volume $v_{\mathrm{A}}/b^3=0.5$ and $v_{\mathrm{C}}/b^3=0.5$). These data are related to Fig 5B.
5. Theoretical calculation of the fraction of A (or C) blocks in the shell vs the logarithm of the transcription rate (rescaled by the rate at which the transcripts are spontaneously incorporated in the paraspeckle). The numbers of segments of A blocks were 5.0 (cyan), 7.5 (orange), 8.15785 (brown), and 8.86482 (black). The parameters used for the calculations are shown in the Materials and Methods (the broken curves are the results for the excluded volume $v_{\mathrm{A}}/b^3=0.5$ and $v_{\mathrm{C}}/b^3=0.5$). These data are related to Fig 6E.