Figure EV1. Transcriptomics of Hdac3 ^−/− cells.

• A
  Anti‐Hdac3 Western blot of naïve Hdac3 ^−/− TNG‐A clones. Specific Hdac3 band is indicated.
• B, C
  Pairwise Pearson correlations similar to Fig 1B, but using different embryo RNAseq samples (Mohammed et al, 2017) (B) or in vitro cell types (Anderson et al, 2017) (D). Primitive streak (PS) Extraembryonic endoderm cell states (nEnd, XEN), anterior definitive endoderm (ADE).
• D
  Similar to Fig 1C with a magnified view of cluster 5. Canonical PrE marker genes are highlighted in red.
• E
  GO terms enriched in cluster 5. Modified Fisher exact P‐values as determined by DAVID (Huang et al, 2008) are shown.
• F–H
  Indicated mRNA levels relative to WT cells of indicated genotypes and treatments after 3 days in SL^RA (F) and 2 days in SL (H). Average and standard deviation (SD) of at least two independent clones. Indicated PrE marker mRNA expression relative to negative control siRNA transfection of cells transfected with indicated siRNAs after 2 days in SL (G).
• I
  Similar to Fig 1C with a magnified view of a panel of naïve and general pluripotency, and post‐implantation markers.

Source data are available online for this figure.