Figure EV1. Knockdown of dpr12 results in regrowth phenotype and description of Dpr12 alleles used in the study, related to Fig 1.

• A
  Left: Graphs depicting the normalized RNA expression levels of selected Dprs in WT γ‐KCs (black), and in γ‐KCs expressing EcR^DN (green) or UNF‐RNAi (blue). *P < 0.05; Error bars indicate SEM; units on the y‐axis are arbitrary. Right: Confocal z‐projections of adult γ‐KCs expressing RNAi transgenes as indicated labeled with membrane‐bound GFP (mCD8‐GFP; CD8) driven by the γ‐specific Gal4 driver R71G10‐Gal4 (γ‐Gal4). Note that while R71G10 is consistently expressed in γ‐KCs, it is also expressed in α/β‐KCs in a stochastic manner.
• B
  A schematic representation of the Dpr12 locus showing introns (black line) and coding and non‐coding exons (red and gray, respectively). The location of Dpr12 gRNA (arrow), dpr12^∆50‐81 mutation (arrow), dpr12 RNAi^JF03210 (black lines connected with dashed lines), and MiMIC^MI01695 (arrowhead) is indicated. SA and SD are splice acceptor and donor sites, respectively. Recombination‐mediated cassette exchange was used to transform Dpr12^MI01695 into Dpr12^GFSTF.
• C
  A schematic representation of Dpr12 protein variants. Signal peptide (SP), Immunoglobulin (Ig), transmembrane (TM), and GPI anchor (GPI).
• D
  Ranking of regrowth: Confocal z‐projections of adult γ‐KCs labeled with membrane‐bound GFP (mCD8‐GFP; CD8) driven by the γ‐specific Gal4 driver GMR71G10‐Gal4 (γ‐Gal4). Representative images of the regrowth defect severity (1 = strong, 2 = intermediate, 3 = weak, 4 = WT) described in Fig 1O. The arrowhead demarcates an unusually short β‐lobe; morphologically abnormal β‐lobes (either short, thin or absent) appear in approximately 40% of either dpr12 or DIP‐δ homozygous mutant brains. Since β‐lobe morphology is rescued by overexpressing a UAS‐Dpr12 transgene within γKCs, it is most likely a non‐cell‐autonomous defect, which is beyond the scope of this study. Asterisk demarcates the distal tip of the γ‐lobe. Green is CD8‐GFP; magenta is FasII staining. Scale bar is 20 µm.