Figure 2. Dpr12 is cell‐autonomously required for γ‐axon regrowth into the γ4/5 zones.

• A–L
  Confocal z‐projections of MARCM neuroblast (NB, A‐F) and single‐cell (SC, G‐L) clones labeled with membrane‐bound GFP (mCD8‐GFP; CD8) driven by the γ‐specific Gal4 driver GMR71G10‐Gal4 (γ‐Gal4). At L3, NB and SC clones expressing dpr12 RNAi are similar to equivalent WT clones (A; n = 20/20, D; n = 15/15, G; n = 15/15 and J; n = 17/17). At 48 h APF and adult stage, WT NB (B; n = 15/15, C; n = 10/10) and SC (H; n = 16/16, I; n = 13/13) clones extend their axons to form the full adult lobe. In contrast, clones expressing dpr12 RNAi (E; n = 14/14, F; n = 22/2, K; n = 18/24, L; n = 19/27) fail to extend their axons to the distal part of the medial lobe (asterisks). (I’ and L’) are traces of multiple single‐cell clones depicting each cell in a different color.
• M
  Top: Schematic representation of WT (orange) and dpr12 RNAi‐expressing (green) single γ‐KC axons. Bottom: Measurements of the relative location to which WT (I) and dpr12 RNAi (L) axons grow across the entire length of the adult γ lobe, alongside the relative position of the proximal border of the γ4 zone (see O, as well as Fig EV2).
• N
  A table depicting the percentage of dpr12 RNAi‐expressing single‐cell clones (SCCs) which stop at the γ3‐γ4 border, at 48 h APF compared to the adult stage.
• O
  Confocal z‐projection of MBONγ4 > γ1γ2 labeled by GMR18H09‐Gal4 driving the expression of mCD8‐GFP (CD8) shown in cyan.

Data information: Yellow arrowheads demarcate single cell bodies. Green, white, and cyan represent mCD8‐GFP. Magenta represents FasII. Scale bar is 20 µm.