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. 2021 Apr 13;40(12):e105763. doi: 10.15252/embj.2020105763

Figure EV4. Characterization of PAM‐DAN and DIP‐δ Gal4s, related to Fig 5.

Figure EV4

  • A–C
    Confocal z‐projections of the cell body region of the PAM‐DAN cluster demonstrating the expression of membrane‐bound tandem tomato (CD4‐tdT; CD4) driven by DIP‐δT2A‐Gal4 (DIP‐δ‐Gal4) in addition to GFP driven by the PAM‐DAN specific QF2 driver GMR58E02‐QF2 (PAM‐QF2). The PAM‐QF2 driver is not expressed at 24 h APF (A, n = 12), starts to be expressed at 48 h APF (B, n = 10) and fully expressed and localized with DIP‐δ‐Gal4 in adult (C, n = 14).
  • D–F
    Confocal z‐projections of heterozygous brains (DIP‐δ+/T2A‐Gal4) in which DIP‐δ positive neurons were labeled by membrane‐bound GFP (mCD8‐GFP; CD8) driven by DIP‐δ‐Gal4. γ‐KCs were marked by expressing membrane‐bound tandem tomato (mtdT‐HA) driven by the γ‐specific QF2 driver GMR71G10‐QF2 (γ‐QF2). Bottom: High magnification images as demarcated by dashed boxes in top panels, of sub‐z‐projections restricted to slices that contain the γ‐lobe.

Data information: Dashed outline demarcates γ‐lobe as depicted by FasII staining. γ4/5 zones are indicated in adult. (D), n = 20; (E), n = 16; (F), n = 26. Scale bar is 20 µm.