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. 2021 Apr 13;40(12):e105763. doi: 10.15252/embj.2020105763

Figure EV5. Phenotypic analysis of PAM‐DAN and MBON innervation of the MB γ‐lobe in Dpr12 mutant brains, related to Fig 7.

Figure EV5

  • A–I
    (A, B, D, E, G, H) Left: Detailed analysis of the confocal z‐projections of dpr12 heterozygous or homozygous mutant brains that are presented in Fig 7A–F, which express membrane‐bound GFP (CD8) driven by: (A‐B) R10G03‐Gal4 is used to label PAM‐DANs innervating the γ4 compartment (PAM‐DAN‐γ4); (D‐E) R48H11‐Gal4 is used to label PAM‐DANs innervating the γ5 compartment (PAM‐DAN‐γ5); (G‐H) R18H09‐Gal4 is used to label the MBONγ4 > γ1γ2 which innervates the γ4 zone (MBON‐γ4). Right: YZ projections along the indicated lines in γ3 (orange) and γ4 or γ5 (blue) compartments. (C, F, I) Top: Cell body numbers of the indicated neurons in dpr12 heterozygous and homozygous brains. Bottom: Summary of innervation destinations. Unknown means stereotypic projections to unidentifiable domains.

Data information: Green is CD8‐GFP, magenta is FasII, and grayscale single channels are shown as indicated. Asterisks mark missing innervation and arrows mark innervations outside the γ lobe. Scale bar is 20 µm in (A‐B, D‐E, G‐H) and 10 µm in (A’‐B’,D’‐E’,G’‐H’).