Figure 6. DIP‐δ is required and sufficient for Dpr12 localization.

• A–J
  Confocal z‐projections of brains expressing MiMIC mediated Dpr12^GFSTF (Dpr12‐GFP) and DIP‐δ^GFSTF (DIP‐δ‐GFP) fusion proteins of the indicated genotypes and time points. (A‐D) Dpr12‐GFP expression is diffuse in DIP‐δ^T2A‐Gal4 homozygotes mutant brains at L3 (A; n = 20/20), 24 h APF (B; n = 14/14), 48 h APF (C; n = 28/28), and adult (D; n = 26/26). (E‐H) DIP‐δ‐GFP expression in dpr12^∆50‐81 homozygotes mutant brains remains localized to the distal part of the γ‐lobe at L3 (E; n = 16/16), 24 h APF (F; n = 16/16), and 48 h APF (G; n = 10/10) but cannot be identified in adult brains (H; n = 16/16). (I, J) Dpr12‐GFP expression in WT animals (I, n = 8/8) or in those ectopically expressing DIP‐δ in PAM‐DANs that innervate the γ3 zone (J, n = 14/14) driven by MB441B‐Gal4 (PAM‐DAN‐γ3‐Gal4). DIP‐δ expression in PAM‐DAN‐γ3 resulted in Dpr12‐GFP localization within the γ3 zone (arrow), in addition to its normal γ4/γ5 localization.

Data information: Arrowheads demarcate DIP‐δ expression at the distal part of the lobe. Asterisks demarcate the distal part of the lobe. Dashed line depicts the medial γ‐lobe, as determined by FasII staining. See legend of Fig EV1D for an explanation regarding the β‐lobe morphological defects observed in (E, J). Green is GFP, and magenta is FasII. Grayscale panels represent single channels, as indicated. Scale bar is 20 µm.