Figure 2.

ABTL0812 induces ER stress in human lung and pancreatic xenografts. Athymic nude mice injected with A549 or MiaPaCa2 cells were treated for 33 d with vehicle or 120 mg/kg ABTL0812 by oral gavage. (a) Tumor xenograft samples were collected and expression of ATF4 or HSPA5 ER stress markers were analyzed by immunoblotting. Left graphs show growth curves of tumors referred to initial values (fold-change ± SEM). Right histograms show the corresponding quantifications referred to ACTB. (b) Expression of DDIT3 protein from MiaPaca2 and A549 tumors was also evaluated by immunohistochemical analysis. Scale bars, 100 µm. Right histogram shows the corresponding quantification. *, P < 0.05; **, P < 0.005; ***, P < 0.001 from vehicle-treated cells, Student’s t-test