Figure 9.

SiNP-induced intracellular stresses contribute to CDK7 and CDK4 activation in NRK cells. (A) NRK cells were pre-incubated with 10 μM CQ for 8 h, and then stimulated by 0.1 μM etoposide for 24 h. The relative expression of (B) LC3-II, (C) CDK4, (D) p-CDK4, (E) CDK7, or (F) p-CDK7 was calculated by the ratio of LC3-II:ACTB, CDK4:ACTB, p-CDK4:ACTB, CDK7:ACTB, or p-CDK7:ACTB, respectively. (G) NRK cells were pre-treated with 10 μM CQ for 8 h, and followed by 0.1 μg/mL tunicamycin treatment for 6 h. The relative expression of (H) LC3-II, (I) CDK4, (J) p-CDK4, (K) CDK7, or (L) p-CDK7 was calculated by the ratio of LC3-II:ACTB, CDK4:ACTB, p-CDK4:ACTB, CDK7:ACTB, or p-CDK7:ACTB, respectively. (M) NRK cells were pre-incubated with 10 μM CQ for 8 h, and then stimulated by 10 μM H₂O₂ for 6 h. The relative expression of (N) LC3-II, (O) CDK4, (P) p-CDK4, (Q) CDK7, or (R) p-CDK7 was calculated by the ratio of LC3-II:ACTB, CDK4:ACTB, p-CDK4:ACTB, CDK7:ACTB, or p-CDK7:ACTB, respectively. * p < 0.05