Figure 2.

Physicochemical properties of the hydrogel. (a) Formation of Cas9/sgRNA assessed by flow cytometery. (b) Formation of SSFH/Cas9/sgRNA assessed by flow cytometery. (c) SSFH diameter size change after Cas9/sgRNA cleavage. (d) Morphologies of SSFH in the absence and presence of Cas9/sgRNA. Photographs show (left) SSFH before addition of Cas9/sgRNA, with the container inverted to show the gel property, and (right) SSFH/Cas9 incubated, with the container inverted to show the loss of gelation. (e) The swelling rate of Cas9/PAH was tested by soaking the lyophilized powder in triple-distilled water. (f) Cas9/sgRNA-edited cutting and release of the SS30 aptamer from SSFH. The cutting and release of the CD123 aptamer from SSFH was tested by gel electrophoresis. SS30, SSFH alone, or SSFH/Cas9 (incubated together for the indicated days) were electrophoresed on an agarose gel.