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. 2021 Jun 12;28(1):1166–1178. doi: 10.1080/10717544.2021.1934191

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Evaluation of binding ability. (a) Flow cytometry assessment of SSFH/Cas9 for CD123 binding specificities. FAM-labeled DNA sequences were incubated with CD123-positive cells Molm-13 and CD123-negative cells RCH-ACV. Fluorescence signal was assessed. (b) Competing assay. Molm-13 cells were incubated with FAM-labeled CD123 antibody and increasing concentrations of free SS30 or SSFH/Cas9. Cells were centrifuged, supernatant fluid were collected and analyzed by fluorometer. The mean fluorescence intensities (MFI) of FAM were analyzed. (c) Confocal microscope assessment of SSFH/Cas9 for CD123 binding specificities.