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Anticancer ability evaluation of SSFH/Cas9 in vitro. (a–c) Proliferation inhibition ability of SSFH/Cas9 to CD123-positive cells. Different concentrations of SSFH/Cas9 was incubated with Molm-13 cells, cell proliferations were detected by MTS assay (a), CCK8 assay (b), and BrdU assay (c) (*p < .05, **p < .01). (d) Cell apoptosis assessment by TUNEL. Molm-13 cells were incubated with free SS30, SSFH, and SSFH/Cas9, respectively. Cell apoptosis were evaluated by TUNEL.
