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. 2020 Feb 17;12(4):273–281. doi: 10.1080/21541248.2020.1728469

Figure 5.

Figure 5.

RAC1 overexpression in zebrafish embryonic development and its treatment with PI3K, AKT and Rho/MRTF/SRF inhibitors. Eight pairs of fish were bred and the resulting embryos were injected with Phenol red or Phenol red + RAC1P29S mRNA during the one-cell stage. 1 μM inhibitors were added at 4 hpf, then removed after 1 h and washed thoroughly. Embryonic morphology was scored at 24 hpf by a blinded observer. (a) Representative images of developmental abnormalities were performed with a Nikon digital sight DS Fi1 camera. Scale bar 100 μm for all images. (b) Embryo phenotypes were scored as normal if they presented an elongated body axis, eye and heart development. The results are shown as mean (SD) of at least three independent experiments (* p < 0.05, ** p < 0.01 versus control)