Figure 1.
MDMs are susceptible to ZIKV infection. Human monocyte-derived macrophages (MDMs) and dendritic cells (moDCs) were uninfected or infected with ZIKVPR or ZIKVU at an MOI of 1.0. At 48 h post-infection (hpi), the cells were fixed with 4% paraformaldehyde and then permeabilized with 0.2% Triton X-100. (A) Viral double-stranded RNA (dsRNA) and (B) envelope (E) protein were identified with J2 and 4G2 antibodies, respectively, and imaged with confocal microscopy. In parallel, MDMs and moDCs were infected with ZIKVPR or ZIKVU at an MOI of 0.1. At 24hpi and 48hpi, ZIKV-infected and mock-infected cells were fixed and stained with J2 or 4G2, and subsequently applied to flow cytometry to determine expression levels of (C) viral dsRNA and (D) E protein. The dot-plot showed data of one representative donor. Statistical analyses in all panels were performed with Student’s t-test and the differences were considered significant when p < 0.05. *p < 0.05, **p < 0.01. ns, not significant. The histogram showed the mean values and standard deviations from three donors. Bars represented 10 μm.