Figure 1.

Schematic representation of experimental protocol. (A) in vivo rat (B) in vitro R28 cell line ischemia and post-C model. (C, D) Representative western blot image and densitometry demonstrating the silencing effect on ATG5 and ATG7 in vivo with intravitreal administration of siRNA. Results were normalized for protein loading using ARRB2. Mean ± SEM; N = 4 per group; * = p < 0.05 for siRNA to Atg5 and Atg7 vs. scrambled. ERG: electroretinogram; OGD: oxygen-glucose deprivation; siRNA Atg: interfering RNA to Atg; WB: western blot