Table 2.
Gene expression of retinal cell markers in R28 cell culture
| Gene Name | Mean CT (n = 8) |
SEM | Fold-change relative to Gapdh |
|---|---|---|---|
| Vim (vimentin) | 18.20 | 0.048 | 0.24 |
| Prkca (protein kinase C, alpha) | 22.94 | 0.098 | 0.009 |
| Nfh (neurofilament heavy chain) | 26.00 | 0.148 | 0.001 |
| Stx1a (syntaxin 1) | 26.14 | 0.0928 | 0.001 |
| Calb1 (calbindin 1) | 31.07 | 0.1928 | 0.00003 |
| Calb2 (calbindin 2) | 30.10 | 0.139 | 0.00006 |
| Tubb3 (tubulin, beta 3 class III) | 30.09 | 0.072 | 0.00006 |
| Gapdh | 16.15 | 0.166 | 1.0 |
| A1cf * | 39.43 | 2.366 | 0.0000001 |
Real-time PCR was performed on R28 cells. Lower mean CT indicates higher levels in R28 cells. The negative control is A1cf and the positive is Gapdh for comparison. Thus Vim, Prkca, Nfh, Stx1a, and Calb1 and Calb2, shown in descending order in the table, are the most abundant retinal neuronal markers in R28 cells under the culture conditions we used. * 3 out of 8 samples had undetectable A1cf.