Table 3.
Primary antibodies used in the study
| Antibody | Manufacturer | Type | Catalog Number | Dilution |
|---|---|---|---|---|
| ATG5 | Santa Cruz Biotechnology | Mouse Monoclonal | sc-133158 | 1:500 |
| ATG7 | Santa Cruz Biotechnology | Mouse Monoclonal | sc-376212 | 1:1000 |
| BECN1 | MBL International | Rabbit Polyclonal | JM-3663-100 | 1:1000 |
| ACTB | Sigma-Aldrich | Mouse Monoclonal | A3854-200UL | 1:25000 |
| ARRB2 | Proteintech | Rabbit Polyclonal | 10171-1-AP | 1:500 |
| TUBB3 | Sigma-Aldrich | Mouse Monoclonal | T8660-.2 ML | 1:250 |
| CALB1 | Proteintech Group | Rabbit Polyclonal | 14479-1-AP | 1:50 |
| CASP3 (cleaved) | Cell Signaling Technology | Rabbit Polyclonal | 9661 S | 1:1000 |
| ITGAM, ITGAX | BD Pharmingen | Mouse Monoclonal | 550299 | 1:500 |
| GFAP | Agilent Technologies | Rabbit Polyclonal | Z033429-2 | 1:1000 |
| LC3-I &II | MBL International | Rabbit Polyclonal | PD014 MS | 1:1000 |
| LC3B | Proteintech Group | Rabbit Polyclonal | 18725-1-AP | 1:500 |
| MTOR | Proteintech Group | Rabbit Polyclonal | 20657-1-AP | 1:500 |
| SQSTM1 | Sigma-Aldrich | Rabbit Polyclonal | p0067 | 1:1000 |
| PRKCA | BD Biosciences | Mouse Monoclonal | 610107 | 1:250/1:500 |
| p-EIF4EBP1/2/3 (Thr45) | Bioss Antibodies | Rabbit Polyclonal | BS-5672 R-A647 | 1:500 |
| RBPMS | GeneTex | Rabbit Polyclonal | GTX118619 | 1:1000 |
| p-RPS6KB1 (Ser421) | Bioss Antibodies | Rabbit Polyclonal | BS-6421RA555 | 1:500 |
| NFH | Covance | Mouse Monoclonal | SMI32-P | 1:250 |
| STX1A | Sigma-Aldrich | Mouse Monoclonal | S0664-.2 ML | 1:250/1:2000 |
| VIM | Santa Cruz Biotechnology | Mouse Monoclonal | sc-6260 | 1:50/1:1000 |
| TH | Iowa Developmental Studies Hybridoma Bank | Mouse Monoclonal | aTH-s | 1:500 |