Fig 2. Screening a chemical library on An. gambiae larvae using the INVAPP / Paragon system.
Concentration response curves for the five compounds in the Medicines for Malaria Venture (MMV) Pathogen Box library identified as active in the primary screen. The movement index for each well was measured before adding the compounds, and then again 240 min later. The second reading is divided by the first to normalize for background variations between the wells caused, for example, by differences in the number of larvae dispensed in each well. The compounds were applied over the range 3.0 × 10−9 M—1.0 × 10−4 M, although only concentrations above 7.8 × 10−7 M are shown for clarity. Larval progeny from both resistant An. coluzzii (Tiassale) and sensitive An. gambiae (G3) strains were studied and labelled “G” and “T” in the figure respectively. Of the compounds tested, only MMV688934 (tolfenpyrad) and MMV637229 (Clemastine) have chemical names.