Table 2. Binding Properties of Various FZD Ligands in Competition with eGFP-WNT-3A Binding (0.4 nM) to HiBiT-FZD₄^a.

	WNT-3A	WNT-5A	WNT-5B	WNT-10B	WNT-11	WNT-16B
Competition binding pKi ± SEM	7.26 ± 0.35	7.08 ± 0.21	6.93 ± 0.25	7.87 ± 0.55	8.90 ± 0.66	n.d.
ΔBRET ± SEM	–0.002 ± 0.001	–0.004 ± 0.0002	–0.004 ± 0.0002	–0.001 ± 0.0003	–0.001 ± 0.0004	n.d.
0.4 nM eGFP- WNT-3A binding displaced (%)	40.8	78.4	78.3	34.5	58.4	n.d.

^aData are based on n = 3–6 individual experiments presented in Figure 3B. pK_i values are presented as a best-fit value ± SEM; n.d. = not determined.